A Secret Weapon For ceesdekker.net
A Secret Weapon For ceesdekker.net
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Rotary motion of DNA rotors on nanopores. Top row: Uncooked video with the Cy5 channel of each rotor. Center row: corresponding single-particle localization success of equally ends on the DNA rotors. The place of the present frame is marked as orange and blue dots, plus the trajectory of the 10 frames ahead of The present body is shown as stable lines.
Human Rad51 filaments on double- and one-stranded DNA: correlating normal and irregular varieties with recombination perform.
Within the similar time, the group of Paul McEuen, who was then based for the University of California, Berkeley, confirmed very similar final results on ropes of bundled nanotubes. According to the wrapping angle, nanotubes can come in metallic or semiconducting variants, a prediction that we and Charles Lieber’s team at Harvard University independently verified employing scanning tunnelling spectroscopy experiments.
Deposition and atomic force microscopy of person phthalocyanine polymers between nanofabricated electrodes
2015, discovery that condensin is often a remarkably flexible protein structure; and initially detection of DNA knots employing nanopores
Example (three) of bending configurations in 3D simulations of DNA rods on nanopores. A portion of the membrane is revealed in grey, the rim from the pore is highlighted in pink, and also a 3D rendering in the movement on the DNA rod is displayed.
Considering the fact that 2000 he moved to one-molecule biophysics and nanobiology, with investigation from DNA supercoiling scientific studies of nucleosomes and DNA restore proteins to DNA translocation through nanopores. A short while ago his exploration has focused on studying mobile division with germs on chip, though his final curiosity is within the direction of knowing artificial cells.
Atomic pressure microscopy reveals that vaccinia topoisomerase IB generates filaments on DNA inside a cooperative vogue
‘You should not just take into consideration the inquiries of tomorrow, and also look at the issues with the working day immediately after tomorrow’
Dekker began his research on solitary carbon nanotubes in 1993 when he create a new line of exploration to review electrical transportation by way of single natural and organic molecules amongst nanoelectrodes. In 1996 a breakthrough was realized with carbon nanotubes. This was realized within a collaboration with the team of Nobel laureate Richard Smalley. STM and nanolithography methods were being accustomed to reveal that these nanotubes are quantum wires at The only-molecule level, with fantastic physical Homes.
Because 2000 he moved to one-molecule biophysics and nanobiology, with investigation from research of DNA loop extrusion and supercoiling to DNA translocation as a result of nanopores. A lot more recently his exploration has focused on finding out chromatin structure and mobile division with germs on chip, though he can be attempting to ultimately Make artificial cells from the bottom up.
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By way of example, we look at the nucleopore complex, a gateway concerning the cell nucleus and also the cytoplasm. This is a really intricate program and thus difficult to review in a very cell, so we use biomimetics. We reconstruct holes and then insert unique aspects in the nucleopore complex to review transport by it at the single-molecule amount.’
It had been an incredibly attention-grabbing time, throughout which I gained a lot of help from Delft College of Technological know-how, exactly where I used to be a professor at enough time, And that i started a number of collaborations with ceesdekker molecular biologists. That aided me study lots.’